Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5139134 | Microchemical Journal | 2017 | 31 Pages |
Abstract
A new procedure developed based on a preconcentration system using a dispersive liquid-liquid microextraction technique based on solidification of floating organic drop (DLLME-SFO) was combined with the molecular absorption spectrometry to determine uranium using 2-(5-bromo-2-pyridylazo)-5-(diethylamino)-phenol (Br-PADAP) as the complexing reagent. The optimization step was performed using two-level factorial design and the Box-Behnken design. The parameters analyzed were pH, Br-PADAP volume, extractant solvent volume and disperser solvent volume. The method allowed determining uranium with a limit of detection of 0.46 μg Lâ 1, limit of quantification 1.53 μg Lâ 1. The enrichment factor was estimated to be 76-fold. The precision expressed as relative standard deviation (RSD) for uranium concentrations of 2 and 10 μg Lâ 1 was 4.91% and 1.85% (n = 15), respectively. Accuracy was assessed using recovery values ranging from 95.28% to 103.86%, which proves that this method does not suffer from a matrix effect and can be satisfactorily applied. The proposed method was applied to determine uranium in human milk samples. Fifteen samples were analyzed, and the uranium concentration ranged from 45.87 ± 3.62 μg Lâ 1 to 160.97 ± 7.00 μg Lâ 1.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Anderson Santos Souza, Rafael Pena Siqueira, Romário Farias Prates, Vanessa Moraes Bezerra, Daniela da Silva Rocha, Márcio Vasconcelos Oliveira, Djanilson Barbosa Santos,