Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5139139 | Microchemical Journal | 2017 | 46 Pages |
Abstract
The present work is aimed at investigating nickel nanoparticles on reduced graphene oxide modified electrode as an amperometric detector along with anion-exchange chromatography. The modified electrode was found to exhibit electrocatalytic oxidation efficiently in relation to amino acids with high stability. The effects of hydroxide and acetate as mobile phase on the retention time, retention factor, separation factor, and peak resolution were subjected to thorough evaluation. The proposed method presented linear ranges from 2.5 Ã 10â 6 to 1.0 Ã 10â 3 mol Lâ 1 with excellent correlation coefficient of at least 0.9997 in optimized anion-exchange chromatographic conditions. The limits of detection for all the compounds analyzed ranged from 8.7 Ã 10â 7 to 2.0 Ã 10â 5 mol Lâ 1. The amperometric sensitivity was found to be within the range of 3.3 Ã 105 and 5.3 Ã 106 nA molâ 1 L. The recovery study showed that the method has good accuracy and repeatability, with recoveries between 95 ± 4.7 and 100 ± 1.7%. Experiment results obtained demonstrated that the sensor has relatively good long-term stability, accuracy, and sensitivity, besides being devoid of interference. The proposed analytical method was applied towards the determination of amino acids in sugarcane vinasse sample obtained from a sugar and ethanol plant.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
José Luiz da Silva, MaÃsa Azevedo Beluomini, Graziela Cristina Sedenho, Nelson Ramos Stradiotto,