Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5139140 | Microchemical Journal | 2017 | 7 Pages |
Abstract
A method of high performance liquid chromatography with UV detection (HPLC-UV) was implemented and validated for formaldehyde determination in bovine milk. A formaldehyde derivatization reaction with 2,4-dinitrophenylhydrazine, at pH 4.0, enabled its detection in milk at 360 nm. The clean up treatment of milk samples by liquid-liquid extraction with low temperature partitioning (LLE-LTP) was thoroughly optimized. The linearity of the method was shown by analytical curves ranging from 10.0 to 400.0 μg Lâ 1 in aqueous solutions and milk samples that were characterized by R2 > 0.99. The limit of quantification of 20.0 μg Lâ 1 demonstrated the high sensitivity of the method to determinate formaldehyde residues in milk. Extracts of milk samples fortified with formaldehyde at three concentration levels, led to a mean overall recovery of 102.2 ± 1.3% (n = 9), which satisfies the performance criteria established by the Codex Alimentarius for analytical methods suitable for determination of food residues. The accuracy was evaluated by comparison with a well-established procedure using the Student t-test. Comparable results were obtained at a 95% confidence level, demonstrating the usefulness and effectiveness of the proposed method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Flavia Borges de Freitas Rezende, Ana Maria de Souza Santos Cheibub, Annibal Duarte Pereira Netto, Flávia Ferreira de Carvalho Marques,