Novel immunochromatographic assay on cotton thread based on carbon nanotubes reporter probe

To perform an assay, in brief, a sandwich immune-complex would form on the test zone in the presence of human ferritin Antigen, and a black band would be observed on the test zone, which indicated the accumulation of CNTs reporter probe on test zone, and can be used as a qualitative mean. Quantitative detection can be realized by recording the color intensity of the test zone with a scanner and “Image J” software.142