| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5141475 | Talanta | 2017 | 28 Pages |
Abstract
A novel sensitive approach for detection of CAP was designed based on CAP specific aptamer and qRT-PCR. The established aptamer-based detection system exhibited a high sensitivity toward CAP with a limit of detection of 0.1Â ng/mL. This aptamer-based detection system also showed high selectivity against interference molecules TAP and FF, which are CAP's structure analogs. Moreover, this detection system was further used to detect CAP in real spiked milk, and the recovery rate of CAP from spiked milk samples ranged from 94.0% to 102.0%.98
Keywords
minimum required performance limitMRPLTCILC-MSdsDNAqRT-PCRSELEXdouble stranded DNAcycle thresholdenzyme linked immunosorbent assayEuropean UnionFluorescence resonance energy transferFRETmilk analysisELISAsystematic evolution of ligands by exponential enrichmentThiamphenicolLOD یا Limit of detectionSpecific recognitionTAPLiquid chromatography mass spectrometryFlorfenicollimit of detectionliquid chromatographyGas chromatographyCaPChloramphenicol
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ye Duan, Lihui Wang, Zhiqiang Gao, Huishan Wang, Hexiao Zhang, Hao Li,