Advanced analytical techniques for fat-soluble vitamin analysis

This review presents advancements in sample preparation and liquid chromatographic methods made during the last 10 years for the fat-soluble vitamin (FSV) analysis in foods. Since activity and bioavailability of organic micronutrients (MOs) depend on the form in which they are present in a food, special emphasis has been given to the most recent techniques, instruments and approaches which have allowed the extraction and separation of various vitamin homologues as well as the comprehensive MO profiling in foods. Due to the numerous ancillary advantages, miniaturized techniques which have been applied in this analysis field are also discussed in this review. A number of selected applications are proposed to enable readers to access the most recent developments and trends aimed at gaining more in-depth knowledge of the vitamin composition of foods.