Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5142389 | Arabian Journal of Chemistry | 2018 | 7 Pages |
Abstract
A new spectrophotometric method for the analysis of creatinine concentration in human serum samples is developed. The method explores the oxidation of p-methylamino phenol sulfate (Metol) in the presence of copper sulfate and creatinine which yields an intense violet colored species with maximum absorbance at 530 nm. The calibration graph of creatinine by fixed time assay ranged from 4.4 to 620 μM. Recovery of creatinine in human serum samples varied from 101% to 106%. Limit of detection and limit of quantification were 0.145 μM and 0.487 μM respectively. Sandell's sensitivity was 0.112 μg cmâ2 and molar absorptivity was 0.101 Ã 104 L molâ1 cmâ1. Within day precision was 2.5-4.8% and day-to-day precision range was 3.2-7.8%. The robustness and ruggedness of the method expressed in RSD values ranged from 0.78% to 2.12% and 1.32% to 3.46% respectively, suggesting that the developed method was rugged. This method provides good sensitivity and is comparable to standard Jaffe's method with comparatively less interference from foreign substances.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Avinash Krishnegowda, Nagaraja Padmarajaiah, Shivakumar Anantharaman, Krishna Honnur,