Short periodicity phase based on ceramide [AP] in the model lipid membranes of stratum corneum does not change during hydration

Small angle X-ray scattering technique was used to determine electron density profiles of short periodicity phase in the model lipid membranes of stratum corneum at different pH. Basic quaternary system was prepared as used previously in the neutron experiments at partial hydration. It was shown that electron density profiles of partially hydrated and fully hydrated model lipid membranes with four basic components were quite similar and demonstrated almost no interbilayer water.