Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5144839 | Ultrasonics Sonochemistry | 2017 | 31 Pages |
Abstract
Here we report the enhancement of both cellular permeability and cell growth rate of Candida tropicalis after treatment with the ultrasonic irradiation of low intensity using a mode of sweeping frequency (UILS) generated by a self-developed ultrasonic device in our lab. After the ultrasonic treatment, remarkable biomass enhancement of the yeast was observed; the hyphae became significantly longer; the seeped cellular protein and nucleic acid from the yeast increased and the cellular Ca2+ content became lower. Illumina transcriptome sequencing showed that the ultrasonic treatment affected the expression of genes involved in diverse cellular components, biological processes and molecular functions. RT-PCR and Western blotting further confirmed the up-/down-regulation of genes in the ultrasound-treated yeasts. The optimal conditions of the ultrasonic treatment for the maximum biomass addition were determined as follows: the yeast was treated for 1 h at the mid logarithmic phase, the frequency was 28 ± 2 kHz and the power density was 120 W/L. Under these conditions, the Candida tropicalis biomass increased by 142.5% compared with the untreated yeast.
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Authors
Guoping Huang, Yingxiu Tang, Ling Sun, Huan Xing, Haile Ma, Ronghai He,