Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5156389 | Carbohydrate Polymers | 2018 | 32 Pages |
Abstract
A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 sâ1 and a half-life time of 111 h at 30 °C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 Ã 109 g·molâ1 that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Marlène Vuillemin, Florent Grimaud, Marion Claverie, Agnès Rolland-Sabaté, Catherine Garnier, Patrick Lucas, Pierre Monsan, Marguerite Dols-Lafargue, Magali Remaud-Siméon, Claire Moulis,