| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5156421 | Carbohydrate Polymers | 2018 | 38 Pages |
Abstract
FosA and InuO showed activity over a broad pH range from 6 to 10 and optimal temperature at 60 °C. Calcium ions and EDTA were found to have no effect on the activity of both enzymes. Kinetic analysis showed that only at relatively low substrate concentrations both enzymes showed Michaelis-Menten type of kinetics for total and transglycosylation activity. Both enzymes showed increased transglycosylation upon increasing substrate concentrations. These are the first examples of the molecular and biochemical characterization of a β-fructofuranosidase (FosA) and an inulosucrase enzyme (InuO) and its product from a Bacillus agaradhaerens strain.
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Authors
Slavko Kralj, Chris Leeflang, EstefanÃa Ibáñez Sierra, BÅażej KempiÅski, Veli Alkan, Marc Kolkman,