Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5156545 | Carbohydrate Polymers | 2017 | 24 Pages |
Abstract
Response surface methodology and central composite design based on single-factor experiments were used to optimize the extraction parameters (i.e., extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from the testa of Salicornia herbacea (STP). The optimal conditions included a liquid-solid ratio of 57:1 (volume/mass), an extraction time of 300 min and an extraction temperature of 87 °C. Under these conditions, the maximal yield of crude STP was 1.30 ± 0.06%, which agreed with model predictions. Preliminary characterization of the purified acidic polysaccharide (STP II-1) by high performance liquid chromatography indicated that it was composed mainly of d-mannose, d-rhamnose, d-glucose, d-galactose, d-arabinose, glucuronic acid and galacturonic acid. The antioxidant capacity of STP II-1 was analyzed by monitoring both the scavenging rate of the 2,2-diphenyl-1-picrylhydrazyl free radical and inhibition rate of H2O2-induced erythrocyte hemolysis.
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Authors
Hongjun Wang, Ziheng Xu, Xinguo Li, Jian Sun, Dan Yao, Hong Jiang, Tiezhong Zhou, Yingzi Liu, Jianguo Li, Chunqiang Wang, Wanbing Wang, Rufeng Yue,