Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5156657 | Carbohydrate Polymers | 2017 | 22 Pages |
Abstract
An acidic heteropolysaccharide, RAPS-1, was isolated from the roots of Rubus anatolicus by water extraction (70 °C) and purification using DEAE-cellulose A52 and Sephadex G-100 column chromatography. The total sugar content and specific optical rotation of RAPS-1 were 96.3% and +196°, respectively. RAPS-1 had a molecular weight of 7900 Da, and was composed of glucose, galactose, and glucuronic acid with a relative molar ratio of 6.2: 1.0: 1.2. Structural features of RAPS-1 were elucidated by a combination of partial acid hydrolysis, periodate oxidation and Smith degradation, methylation, GC-MS, FTIR, and NMR (13C and 1H) analysis. The results indicated that RAPS-1 had a backbone of â4)-α-d-Glcp-(1â residues, with branches attached to O-6 by α-d-Galp-(1â and by α-d-GlcAp-(1â.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Neda Sahragard, Kambiz Jahanbin,