Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5156851 | Carbohydrate Polymers | 2017 | 12 Pages |
Abstract
During prolonged incubation on a solid medium containing sucrose, dextran-producing bacteria showed two distinct phenotypes not related to the genus or species to which they belonged. Colonies of Lc. mesenteroides CM9 from milk and Lb. sakei MN1 from meat formed stable and compact mucoid colonies, whereas the colonies of the other three Leuconostoc strains became diffuse after 72Â h. This differential behaviour was also observed in the ability of the corresponding strains to bind to Caco-2 cells. Strains forming compact mucoid colonies showed a high level of adhesion when grown in the presence of glucose, which decreased in the presence of sucrose (the condition required for dextran synthesis). However no influence of the carbon source was detected for the adhesion ability of the other Lc. mesenteroides strains, which showed variable levels of binding to the enterocytes.
Keywords
DMEMMSELeuconostoc mesenteroidesEPSphorbol 12-myristate 13-acetateCDMSSEC-MALLSHeteropolysaccharidesMRSSLPSHepSCFUPBSHomopolysaccharidesγ˙PMADulbecco’s modified Eagle mediumLABEDTAEthylenediaminetetraacetic acidExopolysaccharidesExopolysaccharideTemLactic acid bacteriaImmunomodulationRheological propertiesDextranPhosphate-buffered salineTransmission electron microscopyShear rateHopsApparent viscosityAdhesionHPLChigh-performance liquid chromatographycolony forming unit
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Kenza Zarour, Mª Goretti Llamas, Alicia Prieto, Patricia Rúas-Madiedo, MarÃa Teresa Dueñas, Pilar Fernández de Palencia, Rosa Aznar, Mebrouk Kihal, Paloma López,