Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5157093 | Carbohydrate Polymers | 2017 | 39 Pages |
Abstract
In this work we have investigated two dextran-producing lactic acid bacteria, Lactobacillus sakei MN1 and Leuconostoc mesenteroides RTF10, isolated from fermented meat products. These bacteria synthesise dextran when sucrose, but not glucose, is present in the growth medium. The influence of dextran on bacterial aggregation, adhesion and biofilm formation was investigated in cultures challenged with sucrose or glucose. For Lb. sakei MN1, the synthesis of the dextran drastically impaired the three processes; in contrast it had no effect on Lc. mesenteroides RTF10. Therefore, the influence of dextran on probiotic properties of Lb. sakei MN1 was tested in vivo using gnotobiotic zebrafish models. The bacterium efficiently colonised the fish gut and inhibited the killing activity of Vibrio anguillarum NB10[pOT11]. Furthermore, under conditions of dextran synthesis, the adhesion of Lb. sakei MN1 to the epithelial cells decreased, without greatly affecting its anti V. anguillarum activity.
Keywords
MRSSFish probioticsPBSCDMSCFUTSBZebrafish modelsDMEMVibrio anguillarumhpiLSDGFPDPFEPSGC–MSDulbecco’s modified Eagle mediumLABExopolysaccharideTemLactic acid bacteriaANOVAone-way analysis of varianceColonisationDextrantrypticase soy brothgas chromatography–mass spectrometryPhosphate-buffered salineTransmission electron microscopyHopsgreen fluorescent proteincolony forming unit
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Montserrat Nácher-Vázquez, Iñaki Iturria, Kenza Zarour, Maria Luz Mohedano, Rosa Aznar, Miguel Ángel Pardo, Paloma López,