Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5157316 | Carbohydrate Polymers | 2017 | 10 Pages |
Abstract
The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy. Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%). Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1 â 3)-glucan. The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2. This revised structure as an acidic β-(1 â 3, 1 â 6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Hui Zhang, Shaoping Nie, Steve W. Cui, Ming Xu, Huihuang Ding, Mingyong Xie,