Novel nano-particulated exopolysaccharide produced by Klebsiella sp. PHRC1.001

In recent decades, microbial synthesis of polysaccharides with special functional properties has attracted increasing attention. This work reported a novel exopolysaccharide (EPS)-producing strain, Klebsiella sp. PHRC1.001 isolated from activated sludge. Physicochemical, rheological, emulsifying and toxicological properties of the obtained EPS were characterized. The EPS was mainly composed of d-glucose and l-arabinose, and was found to exist in aqueous solution in a nano-particulated form (∼50 nm in diameter) with a strong tendency of aggregation. Rheological analysis showed that the EPS aqueous solution was a typical pseudoplastic fluid at higher concentration and could form weak gel upon alkaline treatment followed by neutralization. The EPS exhibited excellent emulsifying properties in stabilizing oil-in-water emulsions presumably by a Pickering mechanism owing to its nanoparticle structure. Acute toxicity test showed that 1.8 g EPS per kg of body weight caused no toxic effect on mice. PHRC1.001 EPS has the potential to be a novel industrial polysaccharide.