| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5164553 | Phytochemistry | 2014 | 11 Pages |
Abstract
Tyrosinase from walnut leaves (Juglans regia) corresponding to the known jrPPO1 sequence was purified and characterized. Two major tyrosinase forms differing only in their C-termini were identified. The first form (jrPPO1(Asp101 â Pro444)) is one amino acid shorter than the second form (jrPPO1(Asp101 â Arg445)).
Keywords
FPLCIEFJuglans regiaPPOACNESI-MSPMSFSDSPEG-4000LC–MS/MSSDS–PAGEUV/Vis spectroscopyAcetonitrileCharge transferisoelectric focusingTyrosinasesodium dodecyl sulfatefast protein liquid chromatographyelectrospray ionisation mass spectrometryMass spectrometryliquid chromatography–mass spectrometry/mass spectrometryphenylmethylsulfonyl fluorideLaccasepolyethylene glycol 4000Polyphenol oxidaseCatechol oxidase
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Florime Zekiri, Christian Molitor, Stephan G. Mauracher, Claudia Michael, Rupert L. Mayer, Christopher Gerner, Annette Rompel,