Effects of N source concentration and NH4+/NO3− ratio on phenylethanoid glycoside pattern in tissue cultures of Plantago lanceolata L.: A metabolomics driven full-factorial experiment with LC-ESI-MS3

Tissue cultures of Plantago lanceolata L. were grown on media with different N source compositions. Phenylethanoid glycosides were quantified by LC-MS, non-linear responses for yields were observed. The full-factorial experimental design and the applied metabolomic approach was shown to be superior to simpler designs with respect to optimization of yields.59