| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5165002 | Phytochemistry | 2013 | 9 Pages |
Abstract
⺠A cDNA of P450 in sanguinarine biosynthesis was isolated from Eschscholzia californica. ⺠Recombinant P450 (CYP82N2v2) was produced in Saccharomyces cerevisiae. ⺠Recombinant CYP82N2v2 showed protopine conversion to produce dihydrosanguinarine. ⺠Recombinant CYP82N2v2 also showed allocryptopine conversion to produce dihydrochelerythrine. ⺠Characterization of the substrate-specificity supported the 6-hydroxylation in the reaction.
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Organic Chemistry
Authors
Tomoya Takemura, Nobuhiro Ikezawa, Kinuko Iwasa, Fumihiko Sato,