Purification, characterization and identification of a senescence related serine protease in dark-induced senescent wheat leaves

A stable senescence related metal-dependent serine protease of the S8A family with specific physical and chemical properties in dark-induced senescent wheat leaves was purified, identified, and characterized. The figure shows the alignment of EP3 protease with serine proteases in other species. The bold lines indicate the peptide fragments identified by MS/MS. Asterisks identified active site residues of this EP3 protease.