| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5165459 | Phytochemistry | 2012 | 7 Pages |
Abstract
⺠Vein Patterning 1-encoded progesterone 5β-reductases are substrate-promiscuous enone reductases. ⺠Catalytically 'strong' and 'weak' progesterone 5β-reductases were identified. ⺠An activity-guided screen was used to propose 'hot spots' for site-directed mutagenesis. ⺠Catalytic efficiency of Vein Patterning 1-encoded progesterone 5β-reductase was improved. ⺠Amino acid residues responsible for progesterone 5β-reductase 'weakness' were substituted.
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Authors
Peter Bauer, Kristin Rudolph, Frieder Müller-Uri, Wolfgang Kreis,