Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5165978 | Phytochemistry | 2011 | 12 Pages |
Abstract
⺠Development of a modified solid medium for quantitative differential proteomics using SILIA. ⺠Establishing an UEB-based protein isolation protocol to increase protein yield and maintain in vivo PTM. ⺠A method for calculating actual incorporation rate of 15N and the whole isotopic envelopes. ⺠Construction of protein standard curves using MS data of 15N/14N-coded peptide pairs. ⺠The protocol established for measurement of in planta protein abundance and PTM is called “SILIA”.
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Authors
Guangyu Guo, Ning Li,