Flavones and phenylpropanoids from a sedative extract of Lantana trifolia L.

The flavones scutellarein-7-O-β-d-apiofuranoside (1), celtidifoline (5,6,4′,5′-tetrahydroxy-7,3′-dimethoxyflavone) (2), and apigenin-7-O-β-d-apiofuranosyl-(1 → 2)-β-d-apiofuranoside (3), were isolated along with other 11 known compounds from leaves of the sedative ethyl acetate extract of Lantana trifolia L. by combined High Speed Countercurrent Chromatography (HSCCC) and HPLC. Their structures were elucidated using 2D NMR spectroscopic and mass spectrometry (ESI-MS) techniques. The sedative properties of L. trifolia extracts were evaluated by the open field method in mice as well as by the affinity for the [3H] flunitrazepam binding sites of the isolated compounds 1-5.