Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5166360 | Phytochemistry | 2009 | 11 Pages |
Abstract
This study defines the regions that contribute to the β-glucosidase aggregating factor (BGAF) binding site of the maize β-glucosidase isozyme Glu1 and provides insights into the mechanism of Glu1-BGAF interaction.
Keywords
PBS4-methylumbelliferyl-β-d-glucosidep-nitrophenyl-β-d-glucosideIPTGFACβ-glucosidaseGel-shift assayisopropyl β-D-1-thiogalactopyranosidePull-down assayProtein–protein interactionbinding siteJacalin-related lectinPhosphate-buffered salinepolymerase chain reactionPCRhigh performance liquid chromatographyHPLCfrontal affinity chromatography
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Hyun Young Yu, Farooqahmed S. Kittur, David R. Bevan, Asim Esen,