Raphanusanin-induced genes and the characterization of RsCSN3, a raphanusanin-induced gene in etiolated radish hypocotyls

This is the first report of genes in radish seedlings induced by the light-induced growth-inhibitory substance raphanusanin. Four known genes encoding functional proteins were found to be differentially expressed in the raphanusanin treated samples versus the lanolin base control. In fact, three of the four genes are genes that respond to diverse environmental stimuli. As a first target of the functional analysis of the candidate genes, we investigated the particularly interesting gene RsCSN3, a homologue of subunit 3 of the COP9 signalosome (CSN3). Expression analysis was used to evaluate the responsiveness of this gene to various stimuli.