Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5166669 | Phytochemistry | 2009 | 8 Pages |
Abstract
A high yield heterologous expression and refolding protocol for recombinant propapain from Escherichia coli inclusion bodies is reported. This recombinant propapain can be activated to mature papain at low pH and its specific activity has been compared with commercially available papain isolated from natural source.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Debi Choudhury, Sumana Roy, Chandana Chakrabarti, Sampa Biswas, J.K. Dattagupta,