Pokeweed antiviral protein region Gly209-Lys225 is critical for RNA N-glycosidase activity of the prokaryotic ribosome

Article ID	Journal	Published Year	Pages	File Type
5166893	Phytochemistry	2008	8 Pages	PDF

Abstract

We investigated the region of the pokeweed antiviral protein (PAP) that contributes to the RNA N-glycosidase activity towards the prokaryotic ribosome by using a series of chimera proteins between PAP and karasurin. Results indicated that the region of PAP (residues 209-225), which was located in the vicinity of the active site, participates in the substrate specificity.

Keywords

Phytolacca americana Pokeweed Phytolaccaceae RNA N-glycosidase Substrate specificity Pokeweed antiviral protein Ribosome-inactivating protein

Related Topics

Physical Sciences and Engineering Chemistry Organic Chemistry

Preview

Pokeweed antiviral protein region Gly209-Lys225 is critical for RNA N-glycosidase activity of the prokaryotic ribosome

Authors

Yoshimi Nagasawa, Kazuyuki Fujii, Takafumi Yoshikawa, Yoshinori Kobayashi, Toshiya Kondo,