Changes in pyridine metabolism profile during growth of trigonelline-forming Lotus japonicus cell cultures

Metabolism of [3H] quinolinic acid and [14C] nicotinamide was monitored in Lotus japonicus cell cultures during growth. The increase in nicotinamide salvage in the lag phase may be due to an increase in activity of nicotinate phosphoribosyltransferase and nicotinamidase, depending primarily on gene expression. Trigonelline formation was particularly pronounced in later growth stages.