Trypsin inhibitors from the garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) seeds: Isolation, characterization and chemical synthesis

Five trypsin inhibitors from the ripe seeds of the garden four-o'clock and spinach were isolated. The primary structures and disulfide bridge pattern were established for natural and chemical synthesized inhibitors. There are probably representatives of a family of serine proteinase inhibitors.