| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5167044 | Phytochemistry | 2007 | 15 Pages |
Abstract
The monitoring of isotope dilution after 13CO2 labelling was optimized using Arabidopsis thaliana Col-0 or Oryza sativa IR57111 plants, which were maximally labelled with 13C. Carbon isotope dilution was evaluated for short (2Â h) and long-term (3 days) kinetic measurements of metabolite pools in roots and shoots. Both approaches were shown to enable the characterization of metabolite specific partitioning processes and kinetics. A current experimental design for the kinetic metabolic phenotyping of higher plants using GC-EI-TOF-MS profiling analysis is proposed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jan Huege, Ronan Sulpice, Yves Gibon, Jan Lisec, Karin Koehl, Joachim Kopka,