Studies on the late steps of (+) pisatin biosynthesis: Evidence for (−) enantiomeric intermediates

The (−) enantiomer of sophorol was more efficiently converted into (+) pisatin than its (+) antipode. Additionally, (−) sophorol reductase (Sor), expressed during synthesis of (+) pisatin, was cloned from pea. The results indicate that intermediates with a (−) chirality are involved in (+) pisatin biosynthesis.