Molecular cloning and heterologous expression of β1,2-xylosyltransferase and core α1,3-fucosyltransferase from maize

A xylosyltransferase (XylT) and a fucosyltransferase (FucT) capable of synthesizing β1,2-xylose or core α1,3-fucose residues on plant complex N-glycans, respectively, have been identified from maize. Heterologously expressed forms of the two enzymes led to the formation of the corresponding N-glycan epitopes on a mammalian glycoprotein.