Characterization in vitro and in vivo of the putative multigene 4-coumarate:CoA ligase network in Arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation

Article ID	Journal	Published Year	Pages	File Type
5168069	Phytochemistry	2005	20 Pages	PDF

Abstract

Detailed characterization of all bona fide At4CL genes, and their corresponding recombinant proteins, together with that of an At4CL5 gene knockout, revealed that formation of syringyl lignin and sinapate ester does not occur via direct ligation of sinapic acid.

Keywords

IPTG The Arabidopsis Information Resource TIGR Cruciferae p-hydroxyphenyl 4CL CWR RT-PCR 4-Coumarate:CoA ligase Arabidopsis thaliana isopropyl-β-d-thiogalactopyranoside syringyl Tair Metabolic networks Lignin The Institute for Genomic Research wild type reverse transcription-polymerase chain reaction guaiacyl

Related Topics

Physical Sciences and Engineering Chemistry Organic Chemistry

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Characterization in vitro and in vivo of the putative multigene 4-coumarate:CoA ligase network in Arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation

Authors

Michael A. Costa, Diana L. Bedgar, Syed G.A. Moinuddin, Kye-Won Kim, Claudia L. Cardenas, Fiona C. Cochrane, Jay M. Shockey, Gregory L. Helms, Yoshiaki Amakura, Hironobu Takahashi, Jessica K. Milhollan, Laurence B. Davin, John Browse, Norman G. Lewis,