Ecdysteroids from the ethanol extract of Diplopterygium rufopilosum

•Three new ecdysteroids were isolated from Diplopterygium rufopilosum.•Two known ecdysteroids were also acquired from the title plant.•The isolated ecdysteroids were evaluated in vitro for antifungal and antibacterial properties.•They all exhibited moderate antibacterial activities against the oral pathogens.

Phytochemical investigation of the ethanol extract of Diplopterygium rufopilosum resulted in the isolation of three new ecdysteroids, (22R,24R,25S,26S)-2β,3β,14α,20R-tetrahydroxy-26α-methoxy-6-oxo-stigmast-7-ene-22,26-lactone (1), (22R,24R,25S)-2β,3β,14α,20R,26S-pentahydroxy-6-oxo-stigmast-7-ene-22,26-lactone (2), and (22R,25S)-2β,3β,14α,20R,24S-pentahydroxy-6,26-dioxo-stigmast-7-ene-22,26-lactone (3), together with two known compounds (4) and (5). Their structures were determined on the basis of spectroscopic analyses, including 1D-NMR, 2D-NMR, and HR-ESI-MS. The isolated ecdysteroids were evaluated in vitro for antimicrobial properties, and exhibited moderate antibacterial activities against the tested oral pathogens.

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