Article ID Journal Published Year Pages File Type
5177391 Phytochemistry Letters 2012 6 Pages PDF
Abstract

A new complex triterpenoid saponin was isolated from the stem bark of Samanea saman by using chromatographic methods. Its structure was established as 3-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]-2,23-dihydroxy-(2β,3β,4α)-olean-12-en-28-oic acid O-β-d-glucopyranosyl-(1 → 3)-O-[O-β-d-glucopyranosyl-(1 → 4)]-O-6-deoxy-α-l-mannopyranosyl-(1 → 2)-6-O-[4-O-[(2E,6S)-2,6-dimethyl-1-oxo-2,7-octadienyl]-6-deoxy-α-l-mannopyranosyl)oxy]-β-d-glucopyranosyl ester (1). Structural elucidation was performed using detailed analyses of 1H and 13C NMR spectra including 2D NMR spectroscopic techniques and chemical conversions. The haemolytic activity of the saponin was evaluated using in vitro assays, and its adjuvant potential on the cellular immune response against ovalbumin antigen was investigated using in vivo models.

Graphical abstractA new triterpenoid saponin was isolated from the stem bark of Samanea saman. Its structure was established by spectroscopic techniques and chemical conversions. The haemolytic potential was evaluated using in vitro assays and its immunological adjuvant activity was investigated using in vivo assays.Download full-size imageHighlights► A new complex triterpenoid saponin was isolated from Samanea saman. ► The structure was established by spectroscopic techniques and chemical conversions. ► The compound exhibited significant immunological adjuvant activity.

Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
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