In-vitro degradation of PLGA nanoparticles in aqueous medium and in stem cell cultures by monitoring the cargo fluorescence spectrum

Additionally, we showed that degradation of up-taken FITC-BSA-PLGA nanoparticles by stem cells may be monitored by the emission spectrum of the released FITC-BSA that shifted towards shorter wavelengths and becomes narrower, after 230 h. Moreover, the changes of fluorescence spectra revealed that the loaded protein is released in the cells during polymer degradation.