Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5203166 | Polymer Degradation and Stability | 2010 | 5 Pages |
Abstract
This study aimed to investigate the factors affecting molecular weight of poly[(R)-3-hydroxybutyrate] [P(3HB)] when polyhydroxyalkanoate (PHA) synthase (PhaRCBsp) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 °C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 Ã 105 to 4.8 Ã 104 with culture time, whereas it was almost unchanged at 25 °C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRCBsp were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCBsp.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Jumiarti Agus, Prihardi Kahar, Manami Hyakutake, Satoshi Tomizawa, Hideki Abe, Takeharu Tsuge, Yasuharu Satoh, Kenji Tajima,