Development and application of a solid phase extraction method for the determination of phthalates in artificial saliva using new synthesised microspheres

Several different parameters, such as pH, adsorption time and adsorbent amount, which affect SPE, were carefully optimised by central composite design (CCD). The best analytical conditions for the simultaneous determination of the analytes from a spiked artificial saliva sample were found to be a pH of 6.8, an adsorption time of 90 min, and an adsorbent amount of 1.8 g L−1, using dichloromethane as the elution solvent. These conditions were applied to study the migration of PEs found on various children's toys into saliva.