Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5210388 | Reactive and Functional Polymers | 2011 | 8 Pages |
Abstract
The semi-interpenetrating polymer network (semi-IPN) nanocomposite hydrogel (NC gel) was successfully synthesized by in situ polymerization of the monomer N-isopropylacrylamide in an aqueous hectorite Laponite suspension containing the polysaccharide alginate (AL). This semi-IPN NC gel reserved thermo-responsibility and high mechanical performance of the PNIPAm-Laponite NC gel. Cell cultures of fibroblasts (L929), human lung adenocarcinoma epithelial cells (A549), and human cervical cancer cells (HeLa) on the semi-IPN NC gels showed improved cell compatibility with addition of AL. By lowering temperature, a rapid cell sheet detachment within 15Â min was observed from the surface of the semi-IPN NC gel containing 0.2Â w/v% of AL without trypsin treatment. The rough surface texture of the semi-IPN NC gel containing AL accelerated the cell sheet detachment, due to faster water penetration. The detached cell sheet was seeded and proliferated again, showing a good cell viability, which indicates that promising applications in tissue engineering and other biomedical fields exist.
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Authors
Tao Wang, Dan Liu, Cuixia Lian, Shudian Zheng, Xinxing Liu, Chaoyang Wang, Zhen Tong,