Effect of gelatin-ionic liquid functional polymers on glucose oxidase and horseradish peroxidase kinetics

The selection of the ionic liquid [emim][EtSO4] and gelatin type A with subsequent maturation of the functional polymer at water activity of 0.76 have been found as the most suitable conditions for the entrapment of both enzymes. The activity of GOD and HRP on the gelatin-[emim][EtSO4] solid disk (6.2 × 1.5 mm of diameter and thickness) formed in the bottom of the well of ELISA plate showed to be respectively 16 times and 13 times lower than in free sodium phosphate buffer. The storage stability at 4 °C of the both immobilized enzymes showed that GOD can retain up to 70% of the initial activity after 2 weeks, where HRP retained 91% of its initial activity. The drop cast deposition of the transparent gelatin-[emim][EtSO4] functional polymer containing the two enzymes (GOD, HRP) and the color-generating precursors on filter paper allowed to demonstrate their applicability on colorimetric glucose detection.