| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5210465 | Reactive and Functional Polymers | 2011 | 6 Pages |
Abstract
Succinylation of OEI-HD-1 at 12% of nitrogens strongly reduced siRNA binding and silencing activity. All other OEI-HD-1 derivates displayed improved in vitro biocompatibility and siRNA mediated luciferase gene knockdown in Neuro2A/eGFPLuc cells. Succinylation at 7% of nitrogens strongly reduced cytotoxicity of the polymer and extended the functional window between gene silencing and cytotoxicity. Incorporation of DMMAn-Mel and PEG into OEI-HD-1 generated a pH-responsive lytic polymer conjugate with the highest potency in siRNA mediated gene knockdown and largest functional activity/biocompatibility window.
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Authors
Alexander Philipp, Martin Meyer, Arkadi Zintchenko, Ernst Wagner,