| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5218514 | Tetrahedron | 2012 | 4 Pages |
Abstract
A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling studies have been performed to understand the effect of the mutations found.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Simon C. Willies, Jemma L. White, Nicholas J. Turner,