| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5219276 | Tetrahedron | 2012 | 7 Pages |
Abstract
A DNA duplex containing an N3-thymidine-butylene-N3-thymidine interstrand cross-link (ICL) was prepared using an on-column orthogonal deprotection strategy to permit different nucleotide sequence composition around the cross-linked site. The conditions used to remove 5â²-O-allyloxycarbonyl and 3â²-O-tert-butyldimethylsilyl protective groups for various on-column oligonucleotide intermediates did not affect the cross-linked lesion. Efficient removal of these groups enabled successful coupling of 2â²-deoxyphosphoramidites to produce the desired duplex with a 31% yield after deprotection and purification.
Graphical abstractDownload full-size image
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Gang Sun, Anne Noronha, Christopher Wilds,