| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5264281 | Tetrahedron Letters | 2013 | 6 Pages |
Abstract
A 1,8-naphthalimide derivative (1) was intentionally designed and synthesized as a new turn-on fluorescent probe for the detection of zinc ion in aqueous media (CH3CN/HEPES, V/VÂ =Â 6:4). Remarkable enhancement of up to 13-fold in fluorescence intensity with a 38Â nm red-shift was achieved in the detection of zinc ion. Compound 1 was successfully applied to the fluorescence imaging of zinc ion, with a fluorescence emission color produced in the cell nucleus different from that produced in the cytoplasm, in A549, BEAS-2B, CHO, Hela, and HepG2 cells. Furthermore, cytokinesis-block micronucleus (CBMN) assay was carried out in CHO cells using 1 and zinc ion as the imaging agents, showing that the 1-Zn2+ agent is a nucleic acid selective stain, which could be applied in MN assays in different kinds of cell lines.
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Authors
Li Yun Zhao, Qi Li Mi, Guang Ke Wang, Jian Hua Chen, Jun Feng Zhang, Qi Hua Zhao, Ying Zhou,