Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5264964 | Tetrahedron Letters | 2014 | 4 Pages |
Abstract
In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510Â nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed that JB interacts with the lipid bilayer at the level of the glycerol moieties.
Related Topics
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Authors
Olimpo GarcÃa-Beltrán, Bruce K. Cassels, Natalia Mena, Marco T. Nuñez, Osvaldo Yañez, Julio Caballero,