Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5268399 | Tetrahedron Letters | 2015 | 4 Pages |
Abstract
Efficient synthesis of 5-[3,6-di(pyridin-2-yl)pyridazine-4-yl]-2â²-deoxyuridine-5â²-O-triphosphate, a potential substrate for fluorescence detection and imaging of DNA is reported. Inverse electron demand Diels-Alder (invDA) reaction between the electron-rich 5-vinyl-2â²-deoxyuridine-5â²-O-triphosphate and electron-deficient tetrazine dienophile 3,6-di(pyridin-2-yl)-1,2,4,5-tetrazine resulted in the formation of 5-[3,6-di(pyridin-2-yl)pyridazine-4-yl]-2â²-deoxyuridine-5â²-O-triphosphate in 80% yield. This class of molecules will find applications in polymerase chain reactions for fluorescence detection and imaging applications of cellular DNA.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Anilkumar R. Kore, Bo Yang, Balasubramanian Srinivasan,