| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5268456 | Tetrahedron Letters | 2010 | 4 Pages |
Abstract
The H-phosphonate of a derivative of thioctic acid (TA) was synthesised and used to introduce a disulfide moiety at the 5â²-end of oligonucleotides. This method overcomes the difficulties experienced with the phosphoramidite approach when employing a cyclic disulfide in the starting alcohol. The disulfide-modified oligonucleotides are subsequently used in metallic nanoparticle (Au and Ag) and surface functionalisation for sensitive, sequence specific analytical detection strategies.
Graphical abstractA convenient route for 5â²-modification of an oligonucleotide with a cyclic disulfide, thioctic acid, is reported using the H-phosphonate method.Download full-size image
Related Topics
Physical Sciences and Engineering
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Authors
Jennifer A. Dougan, Andrew. K. Reid, Duncan Graham,