| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5271317 | Tetrahedron Letters | 2014 | 5 Pages |
Abstract
Fluorogenic aldehydes or probes for monitoring of the progress of aldol reactions have been developed. Fluorescence of benzaldehydes conjugated with aryl groups via a double or triple bond and of their aldol products was evaluated in aqueous solutions. Based on the fluorescence, fluorogenic aldol reaction substrates and retro-aldol reaction substrates were identified. Use of the probe system with optimal fluorescence properties for aldol reactions was demonstrated in assays with purified protein catalysts and with overproduced crude protein catalysts in cell lysates.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Isamu Katsuyama, Pandurang V. Chouthaiwale, Hiroyuki Akama, Hai-Lei Cui, Fujie Tanaka,