| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5272295 | Tetrahedron Letters | 2012 | 4 Pages |
Abstract
An efficient one-step synthesis of a methotrexate fluorescein conjugate is described. Reaction of dried methotrexate with 5-aminofluorescein, mediated by HBTU, produced an isomerically pure methotrexate conjugate in good yield. Proof of structure was achieved by HPLC comparison of materials synthesized by development of a regiospecific pathway employing suitable protecting groups. Either strategy offers a general solution for the preparation of regiospecific methotrexate conjugates.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jonathan Grote, Gangamani Beligere, Sushil Rege,